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Image Search Results
Journal: bioRxiv
Article Title: Cross-reactivity of SARS-CoV structural protein antibodies against SARS-CoV-2
doi: 10.1101/2020.07.30.229377
Figure Lengend Snippet: Immunofluorescence of SARS-CoV-2 structural proteins using SARS-CoV antibodies at 63× magnification. Representative immuno-fluorescence images of HEK 293T cells transiently transfected with SARS-CoV-2 structural proteins. 24 h post-transfection, cells were fixed and stained with the listed SARS-CoV antibodies: (A) Envelope, (B) Membrane, (C) Nucleocapsid, and (D) Spike proteins. All proteins are strep-tagged and control stained with anti-strep-tag antibody or the indicated antigen-specific antibody (Red). DAPI (Blue) was used to visualize cell nuclei.
Article Snippet:
Techniques: Immunofluorescence, Fluorescence, Transfection, Staining, Membrane, Control, Strep-tag
Journal: bioRxiv
Article Title: Cross-reactivity of SARS-CoV structural protein antibodies against SARS-CoV-2
doi: 10.1101/2020.07.30.229377
Figure Lengend Snippet: Biochemical characterization of Spike protein specific antibodies. Characterization of the S-specific antibodies by western blot and ELISA. (A) Coomassie stain of in-house purified His6-tagged RBD protein produced in HEK 293-F suspension cells and purified by Ni-NTA chromatography. (B-D) Western blot of purified RBD protein probed with control α-His6 or 240C. 1 μg purified RBD was loaded for each blot and untransfected HEK 293T control lysate was included to monitor non-specific binding. The 240C antibody was the only antibody to demonstrate binding in western blots (see for details). (E) ELISA on purified full length Spike coated at 2 μg/mL. (F) ELISA on purified RBD coated at 2 μg/mL. (G) Summary table of observed EC50 values from both sets of ELISAs.
Article Snippet:
Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Staining, Purification, Produced, Suspension, Chromatography, Control, Binding Assay
Journal: bioRxiv
Article Title: Cross-reactivity of SARS-CoV structural protein antibodies against SARS-CoV-2
doi: 10.1101/2020.07.30.229377
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Purification, Expressing, Stable Transfection, Plasmid Preparation, Software, Labeling, Enzyme-linked Immunosorbent Assay, Membrane